10035-04-8

Reference

https://en.wikipedia.org/wiki/Calcium_chloride https://pubchem.ncbi.nlm.nih.gov/compound/Calcium_dichloride#section=Information-Sources https://www.drugs.com/pro/calcium-chloride.html

Definition

ChEBI: A hydrate that is the dihydrate form of calcium chloride.

Brand name

Cal Plus (Mallinckrodt).

Biological Functions

Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.Extensive reviews of the experimental measurement of biological calcium have been published. Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.A protocol for the concentration of virus vectors that uses CaCl2 has been published. CaCl2 has been used in the stabilization and twodimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli. The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.

General Description

ACS Reagent Grade.

Biochem/physiol Actions

Calcium chloride act as precipitants during protein crystallization process. It also enhances the competence of Escherichia coli cells as well as strengthened β-lactoglobulin gels by forming crossbridges.

Potential Exposure

Calcium chloride is used as road salt for melting snow, a drying agent in desiccators, for dehydrating organic liquids and gases, in refrigeration brines and antifreeze, as a dust-proofing agent, food additives, concrete hardening accelerator, and others. May react with strong oxidizers.

Shipping

There are no label or maximum shipping quantity requirements set by DOT.

Purification Methods

Crystallise it from ethanol. It is hygroscopic. It loses H2O at 200o so it can be dried at high temperatures to dehydrate it. The hexahydrate [7774-34-7] has m 30o and d 1.67.

Properties and Applications

ITEMS SPECIFICATION APPEARANCE WHITE,HARD ODORLESS FLAKE, POWDER,PELLET,GRANULE CALCIUM CHLORIDE(As CaCl2) 77% min MAGNESIUM&ALKALI METAL SALT (As NaCl) 4.0% max WATER INSOLUBLE MATTER 0.2% max ALKALINITY(As Ca(OH)2) 0.15% max SULFATE (As CaSO4) 0.25% max pH VALUE 7-11 As 5 ppm max Pb 10 ppm max Fe 10 ppm max

ITEMS

SPECIFICATION

APPEARANCE

WHITE,HARD ODORLESS FLAKE, POWDER,PELLET,GRANULE

CALCIUM CHLORIDE(As CaCl2)

77% min

MAGNESIUM&ALKALI METAL SALT (As NaCl)

4.0% max

WATER INSOLUBLE MATTER

0.2% max

ALKALINITY(As Ca(OH)2)

0.15% max

SULFATE (As CaSO4)

0.25% max

pH VALUE

7-11

As

5 ppm max

Pb

10 ppm max

Fe

10 ppm max

Incompatibilities

The solution in water is a weak base. Reacts with zinc in presence of moisture, forming highlyflammable hydrogen gas. Dissolves violently in water with generation of much heat. Incompatible with water, bromine trifluoride; 2-furan, percarboxylic acid. May attack some building materials and metals in the presence of moisture.